1.
Identidiation Of Genetic Susceptiblity Of Myopic Loci In Families From Punjab
by Maria Fareed Siddique | Prof.Dr.Masroor Elahi Babar | Dr. Sehrish Firyal | Prof. Dr. Abu.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2010Dissertation note: Myopia, or nearsightedness, is a condition in which the eye cannot focus on distant objects and sometimes closer ones too. In past different authors reported different loci responsible for myopia. They used specifically synthesized markers for different loci and after conducting linkage analysis through genotyping the myopic families were found to be linked for those loci, whereas, in some studies the cause of myopia was environmental. Till now, linkage studies have identified at least 18 possible loci in 15 different chromosomes associated with myopia, although some of these remain to be confirmed. In past, no study was done in Pakistan on myopic families for finding responsible myopic locus in this regard. So, more conclusive and well-designed studies on family pedigrees of individuals with high myopia were needed to be conducted in Pakistan by using genetic markers associated with myopia.
In this study, a panel of microsatellite markers was developed. Blood samples were taken from six myopic families. DNA was extracted. PCR was performed for amplification of these I microsatellite markers on 34 samples belonging to 6 families. Genotyping analysis was performed for the PCR products of microsatellite markers. These results were studied by constructing and analyzing haplotypes on the basis of PAGE gel bands. Heterozygosity, homozygosity, polymorphism with all microsatellites markers, specific for two loci were checked.
One family MYO-4 was found to be potentially linked with markers for the locus MYP-18. Another family MYO-5 showed potential linkage for the locus 2q37.2. Remaining four families (MYO-l, MYO-2, MYO-3 and MYO-6) were totally unlinked with all the markers (D14S984, D14S63, D14S999, D2S2202, D2S2968 and D2S338 for both loci demonstrating genetic insusceptibility of myopic loci in developing myopia and thus suggesting the complex genetic variability of myopia.
This study will serve as the pioneering database for further research on identifying the genetic heterogenic complexity of myopia. Results of this study lead to development of a panel of microsatellite markers which can be used for linkage studies of more myopic families in Pakistan. This study opens the door for new geneticists as the results can also be helpful in carrying out genetic counseling for the myopic persons who are going to be married and specifically for those who have dominant inheritance. This was a preliminary study on myopic patients in Pakistan and data produced during this study will be helpful for drawing and determining genetic inheritance of expected babies with affected parents and siblings. Moreover this study can become the basis for further research investigations on myopics in Pakistan.
CONCLUSION
This was a pioneering study to develop panel of microsatellite markers for conducting linkage analysis and genetic characterization of myopic patients in Pakistan. As a result of this successful study a reliable, efficient and very informative panel of microsatellite markers was successfully developed which was capable to interpret individual diseased allelic identity, to be used for conducting linkage analysis through genotyping of myopics in Pakistan. This study will serve as the database for further research on identifying the genetic heterogenic complexity of myopia and also these successful results can be further analyzed in future on more myopics from different areas of Pakistan. This work provokes the need for further research purposes in identifying the genes influencing myopia that could help develop targeted treatments for children who are genetically predisposed to developing myopia.
Availability: Items available for loan: UVAS Library [Call number: 1177,T] (1).
2.
Leptin Mutations In Morbidly Obese And Severely Lean Individuals From Pakistan
by Muhammad Wasim | Dr. Sehrish Firyal | Dr. Muhammad | Dr. Muhammad Imran.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1623,T] (1).
3.
Mitochondrial Nd2 Gene Based Molecular Classification Of Various Pakistani Domestic Pigeons
by Muddasar Saeed khan | Dr. Sehrish firyal | Dr. Ali raza awan | DR.Muhammad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1983,T] (1).
4.
Meca Gene Based Methicillin-Resistant Staphylococcus Aureus Typing Isolated From Poultry And Their Potential
by Muhammad Sohaib iftikhar baig | Dr. Sehrish Firyal | Dr. Abu Saeed | Dr. Muhammad Zubair Yousaf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2082,T] (1).
5.
Polymorphisms Of Bovine Tumor Necrosis Factor Alpha Gene And Its Association With Mastitis In Sahiwal Cows
by Huma Sattar (2013-VA-03) | Dr. Sehrish Firyal | Dr. Ali Raza Awan | Dr. Muhammad Tayyab.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Mastitis is one of the shocking maladies of milch animals causing high production losses to livestock industry of Pakistan (Kenyanjui et al. 2011). It is an inflammatory condition of udder; represent a major problem in dairy cow management. It is one of the most common and frequent disease of dairy industry. Producers suffer a huge loss due to veterinary treatment costs and necessary culling of the infected animals. It negatively affects the milk production, quality of milk, and farm economics (Fourichon et al. 2005). Increasing the disease resistance among dairy cattle is therefore desirable because without controlling mastitis, the national goals of developing dairy farming on commercial and scientific lines and production of wholesome milk which conforms to the standards of WTO Accord would remain elusive.
Mastitis is inflammation of udder that caused by physiological and metabolical changes (Schalm and Noorlander 1957). There are two main types of mastitis; clinical mastitis (characterized by classical symptoms i.e., swelling of udder, redness, clumps and clots in milk etc) and sub-clinical mastitis (not show any symptoms, Milk appear normal, udder appear normal) (Schrick et al. 2001). Mastitis is ranked as a top disease of dairy herds (Rinaldi et al. 2010). This mammary gland infection caused by pathogenic micro organisms such as Staphylococcus aureus, Streptococcus uberis, and Esherichia coli in the mammary gland (Heringstad et al. 2000).
India, China and United States are the larger producer of milk and Pakistan is on forth number in milk yield. Pakistan almost produces 36.5 million tons of milk yeild per year (Cady et al. 1983).The Sahiwal breed is well known among for its superior dairy qualities (Barker et al. 1998). Both cross and pure breed Sahiwal cows have high milk production rate (Khan et al. 2013).
It is very difficult to comprehend this disease because numerous environmental and genetic factors are involved in the origin and development of mastitis (Bradley 2002; Carvajal et al. 2013). Susceptibility and resistance to mastitis is a complex trait influenced by genetic variation of animals. Among these variations, the polymorphisms in immunity genes are principal key factors in defensive mechanism of mammary gland (Ibeagha-Awemu et al. 2008).
The mammary gland tissue is protected by immune system by two defense system; innate and acquired immunity. Innate immunity response by the host is a quick response of bacterial defense system (Mesquita et al. 2012). Innate system is a rapid and effective mechanism that activated on recognition of antigen (Akira et al. 2006). Innate immune system is activated when specific pattern recognition receptors (PRR) that are present on the surfaces which are attach to the specific pathogen (Shuster et al. 1996). PRR are presnt on leucocytes in milk and on the epithelial cells lining of udder. It is reported that T- lymphocyte subset i.e., CD4+, CD8+ and ɤδT are present in infected bovine mammary glands. (Goldammer et al. 2004; Strandberg et al. 2005).
Innate defense (nonspecific) of the mammary gland is stimulated by the physical barrier such as teat end, natural killer (NK) cells, neutrophils, macrophages and certain other soluble factors. The teat cannals are considering the main line of defense. Microorganisms enter from teat canal in milk. The main roles of teat sphincter muscles are to remain orifice close so that bacteria cannot enter. This teat canal also lined with keratin, whose estrified and non estified fatty acid function as bacteriostatics that provide protection and play role to eliminate bacteria causing mastitis (Oviedo-Boyso et al. 2007).
If a pathogen is not eliminated by the physical barrier, the acquired immune system is triggered. In comparison, this system is much faster than other immune response. The memory response is significantly stronger, long durable and more efficient to kill the pathogen. The acquired immune system (memory response) have ability to differentiate self or nonself cells and produce antibodies only against antigens through membrane bound protein called major histocompatibility complex (MHC) molecules. Specific immune system activate only when antigens bind with an MHC that is present on the surface of certain cells, this process is referred as antigen presentation. Recognition of pathogenic factors for elimination is mediated by macrophages, several lymphoid, and immunoglobulins (Ig) or antibodies (Sordillo and Streicher 2002).
The most acute responding macrophages and T-cell cytokines are TNF-α, LTF, IL1, IL6, IL8, and IFN-ɤ present in intramammary infection in cows. These genes play important role in improvement of immunity to mastitis (Burton and Erskine 2003).
Tumor necrosis factor alpha is main pro-inflammatory adipokine that is part of systematic immune defense. The main function of TNF-α gene is responsible for proliferation, differentiation and activity of many immune system cells; B lymphocytes, NK (natural killer). It also induces the production and release of many other cytokines (Wojdak Maksymiec et al. 2013) and also enhances the chemotactic and phagocytic effects of immune response. TNF-α gene contains four exons and three introns that are present on chromosome BTA23q22 (Bannerman 2009; Moyes et al. 2009).
TNF-α is a member of a group of cytokines that stimulate the specific immune system. TNF consist of 212 amino acid arranged in stable homotrimers (Kriegler et al. 1988; Tang et al. 1996). The 17-kilodalton (kDa) TNF protomers are composed of two β-pleated sheets and β-strands, joined together antiparallel (Tang et al. 1996).
TNF-α is a component of natural protection systems of humans and animals. Milk gives nourishment and disease resistance to the new born. Various cellular and soluble immune components are important for protecting the mammary gland from infectious diseases like mastitis. Mastitis affects one third of all dairy cows and cost the dairy industry about 2 million dollars annually (National Mastitis Council (1996). Dairy cattle are especially susceptible to mastitis due to diminished mammary gland defense mechanisms (Sordillo and Streicher 2002).
TNF-α is not only produced by activation of macrophages, but also other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. Large amounts of TNF are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1).TNF-α stimulates the proliferation, differentiation and activity of many immune system cells; B lymphocytes, NK (natural killer). TNF-α induces the release of many other cytokines (Wojdak-Maksymiec and Mikolajczyk 2012). TNF-α also enhance the chemotactic and phagocytic effects of immune response.
. The present study is designed to determine the genetic polymorphism in exon 4 of TNF-α gene of mastitic cows and its association resistance and susceptibility towards mastitis.
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6.
Detection And Analysis Of Improvised Explosive Devices Used In Terrorism Activities In Pakistan
by Arslan Nazar (2012-VA-630) | Dr. Sehrish Firyal | Dr. Muhammad Sarwar | Dr. Muhammad Wasim | FaizaMasood.
Material type: ; Literary form:
not fiction
Publisher: 2014Dissertation note: Defence and security organizations are in steadyrequirement of finding new options for the detection of explosives. Fundamental applied research in this area focuses on uncovering of highly energetic substances as well as home-basedexplosives that could be a weapon of mass devastation (Marshal and oxley, 2009 yinon and Zitrin 1996, Scubert and Rimiski-Korsakove, 2006). Current methods of detection for explosives or highly energetic materials are based on a wide variety of technologies that focus on either massexplosives or little portions of explosives. Mass explosives can be distinguishedin some way by imaging features,character of the explosives charge, wires and detonators or unswervingly by spotting the chemistry and dielectric characteristics of the explosive substance. Trace recognition method relay on revealing of vapours given off by the explosives or on explosive’sconstituent part that are set down on nearbyexteriors (national Academy of sciences Committee, 2004). Even though, numerous published material is available about methods of sensing of explosives present in air,water, clothing,soiletc and these put forward the benefit of providing traceconfines of sensation at ppb intensities (Caron et al,. 2010; Hilimi and Luonge). Inthe bulk of the criminal acts, sampling is done at the scene trailed by a sample preparationmove, to be shortly processed by a particular technique for analysis. Sampling and samples preparation are amidmajor, shortcomings in explosive uncovering in many cases frightening the physical condition and life of examiner and the responding officer.
Improvised explosive devices are widely used by military in wars and police to keep up regulation and command. Gush of terrorist activities and increase in criminal conduct have been a matter of great concern worldwide and particularly for Pakistan. There have been motiveless
annihilation of private and community properties as well as industrial centres, causing irretrievabledamages to state and local markets and imperillinghumanexistence(Shen et al. 2005). Potentially perilous explosives like dynamite, varied military explosives havingnitroglycerine (NG), Cyclotrimethylenetrinitramine commonly known as RDX, Cyclotetramethylenetetranitramine and alternativehome-produced low explosives and provocative devices are now currently promptly obtainable to scandalous and terrorists. The haphazard and deliberate uses of these explosives consist ofextortion of cash and taking vengeance, unlawful transportation of prohibited substances, assassinations, terrorist and delinquent activities in numerous regions of the country (Sharma and Lahiri 2005).
Recognition of detonating method, estimating the path ways taken by explosive transportation andarresting the anti-social charactersconnected with unstable materials and explosions is primary aim of explosive analysis. For this purpose various explosive substances and explosive remains are to be examined qualitatively and the ingredients are to be approximated quantitatively using primarily by thin layer chromatography (TLC). TLC is a technique employed for the screening of organic constituents at hand in the post blast samples. The identification of explosives containing alkylammonium nitrate is done by TLC. Secondly Gas chromatography with mass spectrometry (GC-MS) technique with the benefit of anelevated resolving supremacy is avitalapparatus for the analysis of chemical composition of explosives. The extremelyproficient GC analysis withcapillary columns authorizes the examination of explosive hydrocarbons, identical substances of nitroaromatics, hexogen (RDX) and the high explosive pentaerythritoltetranitrate (PETN) in a single run. The spectroscopic identification of explosive materials by FTIR is striking due to the intrinsicpotential of real-time detection, non-vicious analysis, and nominal sample preparation, thirdly the scanning electron microscope (SEM) produces an increased image of the sample based on the contact of an electron beam with the sample’s exterior. Finding of minutemasses of explosive remains play an important part in forces, inhabitant, and counter terrorism requests(Pacheco-Londono et al. 2005). To press on explosives sensor methods, present methods need to become affordable and transportable without disturbing the integrity of the devices. The uncovering of ordinary explosives as well as trinitrotoluene (TNT), RDX, HMX, 2,4,6 Trinitrophenyl-N-methylnitramine (TETRYL)Pentaerythritoltetranitrate (PENT), and NG were carried out using diverseprocedures(Sanchez et al. 2007).
Detection of explosives is anparticularly relevant analytical concern for law enforcement personals and for the environmental protection agencies. As the use of explosive substances have been increased by the terrorists, problems have increased for law enforcement and environment and security agencies regarding the detection ofexplosives residues in baggage, parcels vehicles, aeroplane, on travellers, etc. In bomb scene investigations, it is important to find debris that includes detection of explosive residues. Mobile and hand held explosives detectors, similar to those used for detecting hidden explosives, can be of great help in detecting such residues. Several methods i.e. GC/MS, SEM, FTIR were used in Punjab Forensic Science Agency (PFSA) to analyze residues of explosives. The detection of landmines is an acute, urgent worldwide problem that needs specific and effective detection methods (Yinon 2002). Keeping in mind the above said situations, the project was designed with following objectives
Availability: Items available for loan: UVAS Library [Call number: 2235-T] (1).
